Carcinogenesis, Teratogenesis & Mutagenesis ›› 2004, Vol. 16 ›› Issue (2): 81-82.doi: 10.3969/j.issn.1004-616x.2004.02.004

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Arbitrarily Primed PCR Analysis the Genomic Methylation Pattern in Glioma Cell Induced by NDGA

YAO Li;BIAN Xiu-wu;ZHANG Shu-hui;et al   

  1. Department of pathology，Beijing Medical College of PLA ,Beijing 100071,China
  • Received:2003-09-10 Revised:2003-12-05 Online:2004-03-30 Published:2004-03-30
  • Contact: YAO Li

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Abstract: BACKGROUND ＆ AIM: To investigate the genomic methylation pattern in the differentiation of glioma cell induced by nordihydroguaiaretic acid(NDGA) with methylation－sensitive arbitrarily primed PCR(AP-PCR). MATERIAL AND METHODS:Methylation－sensitive AP-PCR was used to study the genomic methylation changes . RESULTS:The genomic methylation level in SHG－44 cells was increased induced by NDGA. CONCLUSION: Methylation－sensitive arbitrarily primed PCR displayed advantages of detecting genomic methylation pattern.

Key words: methylation－sensitive arbitrarily primed PCR(AP-PCR), genomic methylation, nordihydroguaiaretic acid (NDGA), glioma, differentiation therapy